Molecular characterization of bacteria and archaea in a bioaugmented zero-water exchange shrimp pond

Abstract In the zero-water exchange shrimp culture pond maintained with application of indigenous bioaugmentor, low levels total ammonia–nitrogen were reported, indicating relevance microbial communities. Sediments (0–5 cm layer) sampled from (85th day) and bacterial archaeal communities; specifically, ammonia oxidizers (ammonia-oxidizing bacteria, ammonia-oxidizing archaea, anaerobic bacteria) in sediment metagenome analysed using 16S rRNA functional genes. Bacterial genes showed relative abundance Delta-Proteobacteria Bacteroidetes groups performing sulphur respiration organic matter degradation, respiring Crenarchaeotae , chemolithoautotrophic belonging to Thaumarchaeota . The presence these diverse communities denotes their significant roles cycling carbon, nitrogen, thereby bringing out efficient bioremediation bioaugmented pond. Similarly, gene-specific study predominance Nitrosomonas sp. bacteria), Nitrosopumilus maritimus archaea), Candidatus Kuenenia (anaerobic system, which points importance removal accumulated ammonia. Thus, this paves way for understanding communities, specifically responsible maintaining healthy optimal environmental conditions